endothelial cell of umbilical vein, GM12878, K562
not characterized to standards
WHSC1 (Homo sapiens)
K562GM12878endothelial cell of umbilical vein
- Nuclear lysates from GM12878 (10ug),HUVEC (10ug), K562 (10ug), were loaded into a 4-12% Bis-Tris gel in 1X TA running buffer. After separation, the samples were transferred to a nitrocellulose membrane using iblot. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. The strongest band detected is of the expected size (~157kDa).
- Reviewer comment
- Multiple bands, one of the major bands at indicated size of ~157kD not 50% of signal in entire lane.
- Submitted by
- Noam Shoresh
- Bradley Bernstein, Broad