ENCAB556LSP

Antibody against Homo sapiens WHSC1

Homo sapiens
endothelial cell of umbilical vein, GM12878, K562
not characterized to standards
Status
released
Source (vendor)
Active Motif
Product ID
39879
Lot ID
28612003
Host
mouse
Clonality
monoclonal
Antigen description
Wolf-Hirschhorn syndrome candidate 1; Nuclear SET domain-containing protein 2
Aliases
bradley-bernstein:PchAb 1273
External resources

Characterizations

WHSC1 (Homo sapiens)
K562GM12878endothelial cell of umbilical vein
Method: immunoblot
Attachment from submitter
not compliant
Caption
Nuclear lysates from GM12878 (10ug),HUVEC (10ug), K562 (10ug), were loaded into a 4-12% Bis-Tris gel in 1X TA running buffer. After separation, the samples were transferred to a nitrocellulose membrane using iblot. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. The strongest band detected is of the expected size (~157kDa).
Reviewer comment
Multiple bands, one of the major bands at indicated size of ~157kD not 50% of signal in entire lane.
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad