ENCAB553IRN
Antibody against Homo sapiens PTBP1
Homo sapiens
K562, HepG2
characterized to standards
Homo sapiens
any cell type or tissue
partially characterized
- Status
- released
- Source (vendor)
- MBLI
- Product ID
- RN011P
- Lot ID
- 001
- Characterized targets
- PTBP1 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Antigen description
- Peptide, 46-59 aa
- Antigen sequence
- KFKGDSRSAGVPSR
- Aliases
- xiang-dong-fu:PTBP1
- External resources
Characterizations
PTBP1 (Homo sapiens)
HepG2
Method: immunoprecipitation
compliant
- Caption
- IP-Western Blot analysis of HepG2 whole cell lysate using PTBP1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-PTBP1 antibody (lanes under 'PTBP1').
- Submitted by
- Steven Blue
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- HepG2_MBL_RN011P_001_PTBP1.png
PTBP1 (Homo sapiens)
K562
Method: immunoprecipitation
compliant
- Caption
- IP-Western Blot analysis of K562 whole cell lysate using PTBP1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-PTBP1 antibody (lanes under 'PTBP1').
- Submitted by
- Steven Blue
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- K562_MBLI_RN011P_001_PTBP1.png
PTBP1 (Homo sapiens)
Method: immunoprecipitation
not submitted for review by lab
- Caption
- IP-WB analysis of K562 whole cell lysate using PTBP1 specific antibody. Lane 1 is 2.5% of 0.5mg input lysate, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal Anti-PTBP1(Human)pAb. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
- Submitted by
- Balaji Sundararaman
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- MBLI_RN011P_001_PTBP1.png
PTBP1 (Homo sapiens)
Method: knockdown or knockout
compliant
- Caption
- Western blot following CRISPR against PTBP1 in K562 whole cell lysate using PTBP1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against PTBP1. PTBP1 protein appears as the green band, GAPDH serves as a control and appears in red.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005
- Download
- PTBP1-K562-CRISPR.png
PTBP1 (Homo sapiens)
Method: knockdown or knockout
compliant
- Caption
- Western blot following shRNA against PTBP1 in K562 and HepG2 whole cell lysate using PTBP1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against PTBP1. Lanes 5-7 follow the same pattern, but in HepG2. PTBP1 protein appears as the green band, GAPDH serves as a control and appears in red.
- Submitted by
- Brenton Graveley
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005
- Download
- PTBP1_Secondary_Western.png