1. Antibodies
  2. Homo sapiens
  3. NBN

ENCAB543ANT

Antibody against Homo sapiens NBN

Homo sapiens
K562, MCF-7, HepG2, GM12878
characterized to standards
Status
released
Source (vendor)
GeneTex
Product ID
GTX103229
Lot ID
41101
Characterized targets
NBN (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Aliases
michael-snyder:AS-1219
External resources

Characterizations

NBN (Homo sapiens)
GM12878
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878, using the antibody GTX103229. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 84.959
Reviewer comment
Multiple bands, but marked band >50% of total signal in lane.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
NBN (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody GTX103229. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 84.959
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
NBN (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody GTX103229. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 84.959
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
Download
 expt1046_3.jpg
NBN (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody GTX103229. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 84.959
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
NBN (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody GTX103229. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 84.959.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
Download
 NBN.JPG
NBN (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
compliant
Caption
IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using the antibody GTX103229, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitter comment
Genecard: Rad50 is component of the MRN complex composed of two heterodimers RAD50/MRE11A associated with a single NBN. And rad50 is component of the BASC complex, at least composed of BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50, MRE11A and NBN, http://www.genecards.org/cgi-bin/carddisp.pl?gene=RAD50&keywords=rad50. MRE11A's explanation is same.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford