1. Antibodies
  2. Homo sapiens
  3. HIST2H3A + HIST2H3D + HIST2H3C

ENCAB539BDB

Antibody against Homo sapiens H3K4me1

Homo sapiens
any cell type or tissue
characterized to standards
Status
released
Source (vendor)
Abcam
Product ID
ab8895
Lot ID
GR43807-1
Characterized targets
H3K4me1 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Aliases
bradley-bernstein:PchAb 400
External resources

Characterizations

H3K4me1 (Homo sapiens)
K562HEK293
Method: immunoblot
Attachment from submitter
Attachment from submitters
compliant
Caption
0.25ug unmodified Recombinant Histone H3 (Millipore 14-494) and nuclear extract from 100,000 HEK293 andK562 cells were resolved by electrophoresis on a 4-12% acrylamide gel. After separation, the samples were transferred to a nitrocellulose membrane with an Invitrogen iBlot system. Membrane was blocked for an hour in room temperature, with 5% nonfat dry milk and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. Panel A: anti Histone H3 (Abcam ab1791), band of expected size (~17kDa) visualized in both unmodified recombinant histone H3 and NL, at similar intensities.Panel B: Band of expected size (~17kDa) visualized in Nuclear lysates, and cannot be detected in unmodified recombinant Histone H3. Antibody seems to be specific since no reactivity with nonhistone proteins or unmodified histones was detected
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad
H3K4me1 (Homo sapiens)
Method: dot blot assay
Attachment PDF Icon
compliant
Caption
We performed a DB analysis using the large set of modified peptides provided in the Active Motif MODified Histone Peptide Array, catalog #13005. The only peptide recognized by the H3K4me1 antibody was the correct one. This replaces a previous DOT BLOT.
Submitter comment
Please replace existing dot blot image with this updated characterization
Submitted by
Nina Farrell
Lab
Bradley Bernstein, Broad
H3K4me1 (Homo sapiens)
Method: dot blot assay
Attachment from submitter
Attachment from submitters
compliant
Caption
Performed dot blot analysis using a large set of modified peptides. The peptide which showed the highest signal indicating recognition by the H3K4me1 antibody was the correct one.
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad