ENCAB511DBP
Antibody against Homo sapiens FIP1L1
Homo sapiens
HepG2, K562
characterized to standards
- Status
- released
- Source (vendor)
- ABclonal
- Product ID
- A5016
- Lot ID
- unknown
- Characterized targets
- FIP1L1 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Isotype
- IgG
- Antigen description
- Recombinant protein of human FIP1L1
- Aliases
- xiang-dong-fu:FIP1L1
- External resources
Characterizations
FIP1L1 (Homo sapiens)
Method: knockdown or knockout
compliant
- Caption
- Western blot following shRNA against FIP1L1 in K562 and HepG2 whole cell lysate using FIP1L1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against FIP1L1. Lanes 5-8 follow the same pattern, but in HepG2. FIP1L1 protein appears as the green band, Tubulin serves as a control and appears in red.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005
- Download
- FIP1L1-FU'S_Secondary_Western.png
FIP1L1 (Homo sapiens)
HepG2
Method: immunoprecipitation
compliant
- Caption
- IP-Western Blot analysis of HepG2 whole cell lysate using FIP1L1 specific antibody. Lane 1 is 0.5% of ten million whole cell lysate input (lane under '10% input') , lane 2 is 5% of IP enrichment using rabbit normal IgG (lane under 'IgG') and lane 3 is 5% IP enrichment using rabbit polyclonal anti-FIP1L1 antibody (lanes under 'anti-FIP1L1'). Asterisk indicates heavy chain of antibody.
- Submitted by
- Marcus Ho
- Lab
- Xiang-Dong Fu, UCSD
- Grant
- U54HG007005
- Download
- FIP1L1_HepG2.png
FIP1L1 (Homo sapiens)
K562
Method: immunoprecipitation
compliant
- Caption
- IP-Western Blot analysis of K562 whole cell lysate using FIP1L1 specific antibody. Lane 1 is 0.5% of ten million whole cell lysate input (lane under '10% input') , lane 2 is 5% of IP enrichment using rabbit normal IgG (lane under 'IgG') and lane 3 is 5% IP enrichment using rabbit polyclonal anti-FIP1L1 antibody (lanes under 'anti-FIP1L1'). Asterisk indicates heavy chain of antibody.
- Submitted by
- Marcus Ho
- Lab
- Xiang-Dong Fu, UCSD
- Grant
- U54HG007005
- Download
- FIP1L1_K562.png