ENCAB509XJY
Antibody against Homo sapiens CHD3
Homo sapiens
HepG2, K562
characterized to standards
- Status
- released
- Source (vendor)
- Zen Bioscience
- Product ID
- 200314-7F6
- Lot ID
- CD1016
- Characterized targets
- CHD3 (Homo sapiens)
- Host
- mouse
- Clonality
- monoclonal
- Isotype
- IgG1
- Antigen description
- Purified recombinant human CHD3 (C-terminus) protein fragments expressed in E.coli
- Aliases
- xiang-dong-fu:CHD3
- External resources
Characterizations
CHD3 (Homo sapiens)
Method: knockdown or knockout
compliant
- Caption
- Western blot following CRISPR against CHD3 in HepG2 whole cell lysate using CHD3 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CHD3.CHD3 protein appears as the green band, Tubulin serves as a control and appears in red.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U41HG009889
- Download
- CHD3-HEPG2-CRISPR.png
CHD3 (Homo sapiens)
HepG2
Method: immunoblot
compliant
- Caption
- IP-Western Blot analysis of HepG2 whole cell lysate using CHD3 specific antibody. Lane 1 is 1% of ten million whole cell lysate input (lane under '5% input') , lane 2 is 20% of IP enrichment using mouse normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using mouse monoclonal anti-CHD3 antibody (lanes under 'anti-CHD3').
- Submitted by
- Marcus Ho
- Lab
- Xiang-Dong Fu, UCSD
- Grant
- U54HG007005
- Download
- CHD3_HepG2_revised.png
CHD3 (Homo sapiens)
K562
Method: immunoprecipitation
compliant
- Caption
- IP-Western Blot analysis of K562 whole cell lysate using CHD3 specific antibody. Lane 1 is 2% of ten million whole cell lysate input (lane under '10% input') , lane 2 is 20% of IP enrichment using mouse normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using mouse monoclonal anti-CHD3 antibody (lanes under 'CHD3').
- Submitted by
- Rui Xiao
- Lab
- Xiang-Dong Fu, UCSD
- Grant
- U54HG007005
- Download
- CHD3_K562.png