ENCAB502MPG

Antibody against Homo sapiens HNRNPU

Homo sapiens
HepG2, K562
characterized to standards
Homo sapiens
adrenal gland
characterized to standards with exemption
Status
released
Source (vendor)
Santa Cruz Biotech
Product ID
sc-32315
Lot ID
C3012
Characterized targets
HNRNPU (Homo sapiens)
Host
mouse
Clonality
monoclonal
Purification
affinity
Isotype
IgG1
Antigen description
hnRNP U (3G6) is a mouse monoclonal antibody raised against recombinant full length protein of hnRNP U of human origin.
External resources

Characterizations

HNRNPU (Homo sapiens)
adrenal gland
Method: immunoprecipitation
Attachment from submitter
exempt from standards
Caption
IP-Western Blot analysis of human adrenal gland lysate using HNRNPU specific antibody. Lane 1 is 1% of 10 million tissue lysate input and lane 2 is 10% of IP enrichment using rabbit polyclonal anti-HNRNPU antibody (lanes under 'HNRNPU'). Lanes 3 and 4 indicate a technical replicate of lanes 1 and 2.
Submitter comment
There was not enough adrenal tissue to full characterize this antibody in this specific tissue type.
Reviewer comment
This antibody is compliant for adrenal tissues as part of the GTex project. As there is a limited amount of tissue, no IgG control or secondary KD will be done on adrenal tissues with this antibody, but it has compliant primary and secondary characterizations in multiple cell lines as well, and is therefore exempt from standards.
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD
HNRNPU (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
IP-Western Blot analysis of HepG2 whole cell lysate using HNRNPU specific antibody. Lane 1 is 2.5% of 0.5mg input lysate, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using either mouse monoclonal hnRNP U antibody (lanes under 'HNRNPU') or using mouse normal IgG (lanes under 'IgG').
Submitted by
Balaji Sundararaman
Lab
Gene Yeo, UCSD
HNRNPU (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
compliant
Caption
Western blot following shRNA against HNRNPU in K562 and HepG2 whole cell lysate using HNRNPU specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against HNRNPU. Lanes 5-8 follow the same pattern, but in HepG2. HNRNPU protein appears as the green band, Tubulin serves as a control and appears in red.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
HNRNPU (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
IP-WB characterization of HNRNPU specific antibody in K562 cell line . Lane 1 is 2.5% of five million K562 whole cell lysate Input, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using mouse monoclonal hnRNP U Antibody (3G6). This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
Submitted by
Balaji Sundararaman
Lab
Gene Yeo, UCSD