ENCAB498PCM

Alternate accession: ENCAB323IWF

Antibody against Homo sapiens CTCF

Homo sapiens
RWPE1, HepG2, HCT116, LNCAP, GM12878, HeLa-S3, C4-2B, VCaP, MCF-7, K562, 22Rv1, Panc1, RWPE2
characterized to standards
Homo sapiens
epithelial cell of prostate
characterized to standards with exemption
Status
released
Source (vendor)
Cell Signaling
Product ID
3418
Lot ID
1
Characterized targets
CTCF (Homo sapiens)
Host
rabbit
Clonality
monoclonal
Isotype
IgG
Antigen description
Insulators, chromatin boundaries. Rabbit mAb detects endogenous levels of total CTCF protein. This antibody does not cross-react with BORIS and was produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of the human CTCF protein.
Aliases
michael-snyder:AS-1630, bradley-bernstein:PchAb 1203
External resources

Characterizations

CTCF (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
compliant
Caption
"Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® CTCF siRNA I (+), using CTCF (D31H2) XP® Rabbit mAb #3418 (upper) or -Tubulin (11H10) Rabbit mAb #2125 (lower). The CTCF (D31H2) XP® Rabbit mAb confirms silencing of CTCF expression, while the -Tubulin (11H10) Rabbit mAb is used as a loading control."
Submitter comment
This characterization was provided by the vendor for lot 1 of this antibody. We submitted it to the ENCODE Antibody Review Panel and it was allowed to be used as a supporting siRNA knockdown charcterization.
Submitted by
Jessika Adrian
Lab
Michael Snyder, Stanford
CTCF (Homo sapiens)
Panc1
Method: immunoblot
compliant
Caption
Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order: Panc1 using the antibody CST_3418S. Molecular Weight: 140.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
CTCF (Homo sapiens)
epithelial cell of prostate
Method: immunoprecipitation
Attachment from submitter
exempt from standards
Caption
The prostate epithelial cells were limiting and no more were available for primary characterizations.
Submitter comment
These prostate epithelial cells were limiting and we do not have more to use for antibody characterizations.
Reviewer comment
An exemption was made by the antibody review panel on June 19, 2017 under subsection a) "Additional situations for Primary Characterization", as these primary cells were limiting and no more were available for antibody characterizations.
Submitted by
Heather Witt
Lab
Peggy Farnham, USC
CTCF (Homo sapiens)
GM12878HCT116HeLa-S3HepG2K562MCF-7
Method: immunoblot
Attachment from submitter
compliant
Caption
Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order: GM12878, HCT116, HeLa-S3, HepG2, K562, MCF-7 using the antibody CST_3418S. Molecular Weight: 140
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
CTCF (Homo sapiens)
C4-2BLNCAPRWPE1RWPE2VCaP22Rv1
Method: immunoblot
compliant
Caption
Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order: C4-2B,LNCaP,RWPE-1,RWPE-2,VCaP,22Rv1,CNON SEP076,CNON SEP026,CNON SEP076 using the antibody CST_3418S. Molecular Weight: 140.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford