ENCAB495WEF
Alternate accession: ENCAB802IQW
Antibody against Homo sapiens SMARCA5
Homo sapiens
K562, GM12878
partially characterized
Homo sapiens
HepG2
not characterized to standards
- Status
- released
- Source (vendor)
- Bethyl Labs
- Product ID
- A301-018A
- Lot ID
- 1
- Characterized targets
- SMARCA5 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Isotype
- IgG
- Aliases
- bradley-bernstein:PchAb 1242, michael-snyder:AS-1284
- External resources
Characterizations
SMARCA5 (Homo sapiens)
K562HepG2GM12878
compliant
- Caption
- Nuclear lysates from K562(10ug), GM12878 (7ug), HepG2 (10ug), were loaded into a 4-12% Bis-Tris gel in 1X MOPS running buffer. After separation, the samples were transferred to a PVDF membrane using wet transfer. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. a stronger band of expected size was detected (~120kDa).
- Submitted by
- Noam Shoresh
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991