ENCAB495WEF

Alternate accession: ENCAB802IQW

Antibody against Homo sapiens SMARCA5

Homo sapiens
K562, GM12878
partially characterized
Homo sapiens
HepG2
not characterized to standards
Status
released
Source (vendor)
Bethyl Labs
Product ID
A301-018A
Lot ID
1
Host
rabbit
Clonality
polyclonal
Purification
affinity
Isotype
IgG
Aliases
bradley-bernstein:PchAb 1242, michael-snyder:AS-1284
External resources

Characterizations

SMARCA5 (Homo sapiens)
K562HepG2GM12878
Method: immunoblot
Attachment from submitter
compliant
Caption
Nuclear lysates from K562(10ug), GM12878 (7ug), HepG2 (10ug), were loaded into a 4-12% Bis-Tris gel in 1X MOPS running buffer. After separation, the samples were transferred to a PVDF membrane using wet transfer. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. a stronger band of expected size was detected (~120kDa).
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad