ENCAB483CQL
Antibody against Homo sapiens USF2
Homo sapiens
IMR-90, A549, SK-N-SH, K562
characterized to standards
- Status
- released
- Source (vendor)
- Abcam
- Product ID
- ab60931
- Lot ID
- GR236233
- Characterized targets
- USF2 (Homo sapiens)
- Host
- mouse
- Clonality
- monoclonal
- Isotype
- IgG2a
- Antigen description
- Recombinant fragment with tag: MDMLDPGLDP AASATAAAAA SHDKGPEAEE GVELQEGGDG PGAEEQTAVA ITSVQQAAFG DHNIQYQFRT ETNGGQVTYR VVQVTDGQLD GQGDTAGAVS , corresponding to amino acids 1-101 of Human USF2
- Aliases
- michael-snyder:AS-1664
- External resources
Characterizations
USF2 (Homo sapiens)
A549
compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: A549 using the antibody ab60931 (Lot: GR236233). The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 37.
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- #1146 A549 USF2(ab60931).jpg
USF2 (Homo sapiens)
IMR-90
compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: IMR90 using the antibody ab60931 (Lot: GR236233). The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 37.
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- #1146 IMR90 USF2(ab60931).jpg
USF2 (Homo sapiens)
SK-N-SH
compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: SK-N-SH using the antibody ab60931 (Lot: GR236233). The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 37.
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- IP1148 SK-N-SH USF2 ab60931.jpg
USF2 (Homo sapiens)
K562
compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody ab60931 (Lot: GR236233). Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 37.
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- MS1040_4_USF2-ab60931.JPG
USF2 (Homo sapiens)
compliant
- Caption
- IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using the antibody ab60931 (Lot: GR236233), and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
- Submitter comment
- All of higher ranked proteins are not DNA binding TFs.
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- USF2_ab60931 final.pdf