ENCAB483CQL

Antibody against Homo sapiens USF2

Homo sapiens
K562, SK-N-SH, IMR-90, A549
characterized to standards
Status
released
Source (vendor)
Abcam
Product ID
ab60931
Lot ID
GR236233
Characterized targets
USF2 (Homo sapiens)
Host
mouse
Clonality
monoclonal
Isotype
IgG2a
Antigen description
Recombinant fragment with tag: MDMLDPGLDP AASATAAAAA SHDKGPEAEE GVELQEGGDG PGAEEQTAVA ITSVQQAAFG DHNIQYQFRT ETNGGQVTYR VVQVTDGQLD GQGDTAGAVS , corresponding to amino acids 1-101 of Human USF2
Aliases
michael-snyder:AS-1664
External resources

Characterizations

USF2 (Homo sapiens)
A549
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: A549 using the antibody ab60931 (Lot: GR236233). The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 37.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
USF2 (Homo sapiens)
IMR-90
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: IMR90 using the antibody ab60931 (Lot: GR236233). The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 37.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
USF2 (Homo sapiens)
SK-N-SH
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: SK-N-SH using the antibody ab60931 (Lot: GR236233). The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 37.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
USF2 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody ab60931 (Lot: GR236233). Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 37.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
USF2 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
compliant
Caption
IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using the antibody ab60931 (Lot: GR236233), and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitter comment
All of higher ranked proteins are not DNA binding TFs.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford