ENCAB480FQK
Antibody against Homo sapiens EXOSC5
Homo sapiens
K562, HepG2
characterized to standards
Homo sapiens
any cell type or tissue
partially characterized
- Status
- released
- Source (vendor)
- MBLI
- Product ID
- RN100PW
- Lot ID
- 001
- Characterized targets
- EXOSC5 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- External resources
Characterizations
EXOSC5 (Homo sapiens)
Method: knockdown or knockout
compliant
- Caption
- Western blot following CRISPR against EXOSC5 in HepG2 whole cell lysate using EXOSC5 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EXOSC5.EXOSC5 protein appears as the green band, Tubulin serves as a control and appears in red.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005
- Download
- EXOSC5-CRISPR-HEPG2.png
EXOSC5 (Homo sapiens)
Method: knockdown or knockout
compliant
- Caption
- Western blot following CRISPR against EXOSC5 in K562 whole cell lysate using EXOSC5 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EXOSC5.EXOSC5 protein appears as the green band, Tubulin serves as a control and appears in red.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005
- Download
- EXOSC5-CRISPR-K562.png
EXOSC5 (Homo sapiens)
HepG2
Method: immunoprecipitation
compliant
- Caption
- IP-Western Blot analysis of HepG2 whole cell lysate using EXOSC5 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-EXOSC5 antibody (lanes under 'EXOSC5').
- Submitted by
- Steven Blue
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- HepG2_MBLI_RN100PW_001_EXOSC5.png
EXOSC5 (Homo sapiens)
K562
Method: immunoprecipitation
compliant
- Caption
- IP-Western Blot analysis of K562 whole cell lysate using EXOSC5 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-EXOSC5 antibody (lanes under 'EXOSC5').
- Submitted by
- Steven Blue
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- K562_MBLI_RN100PW_001_EXOSC5.png
EXOSC5 (Homo sapiens)
Method: immunoprecipitation
not submitted for review by lab
- Caption
- IP-WB analysis of K562 whole cell lysate using EXOSC5 specific antibody. Lane 1 is 2.5% of five million whole cell lysate Input, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal Anti-EXOSC5 (RRP46) (Human) pAb. This antibody did not meet our primary validation criteria using our standard IP protocol in the indicated cell type.
- Submitted by
- Balaji Sundararaman
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- MBLI_RN100PW_001_EXOSC5.png