ENCAB480FQK

Antibody against Homo sapiens EXOSC5

Homo sapiens
HepG2, K562
characterized to standards
Homo sapiens
any cell type or tissue
partially characterized
Status
released
Source (vendor)
MBLI
Product ID
RN100PW
Lot ID
001
Characterized targets
EXOSC5 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
External resources

Characterizations

EXOSC5 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
compliant
Caption
Western blot following CRISPR against EXOSC5 in HepG2 whole cell lysate using EXOSC5 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EXOSC5.EXOSC5 protein appears as the green band, Tubulin serves as a control and appears in red.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
EXOSC5 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
compliant
Caption
Western blot following CRISPR against EXOSC5 in K562 whole cell lysate using EXOSC5 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EXOSC5.EXOSC5 protein appears as the green band, Tubulin serves as a control and appears in red.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
EXOSC5 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
IP-Western Blot analysis of HepG2 whole cell lysate using EXOSC5 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-EXOSC5 antibody (lanes under 'EXOSC5').
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD
EXOSC5 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
IP-Western Blot analysis of K562 whole cell lysate using EXOSC5 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-EXOSC5 antibody (lanes under 'EXOSC5').
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD
EXOSC5 (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Caption
IP-WB analysis of K562 whole cell lysate using EXOSC5 specific antibody. Lane 1 is 2.5% of five million whole cell lysate Input, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal Anti-EXOSC5 (RRP46) (Human) pAb. This antibody did not meet our primary validation criteria using our standard IP protocol in the indicated cell type.
Submitted by
Balaji Sundararaman
Lab
Gene Yeo, UCSD