ENCAB472HKJ

Antibody against Homo sapiens H3K4me1

Homo sapiens
any cell type or tissue
characterized to standards
Status
released
Source (vendor)
Millipore
Product ID
07-436
Lot ID
2721669
Characterized targets
H3K4me1 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Aliases
bradley-bernstein:PchAb 1359
External resources

Characterizations

H3K4me1 (Homo sapiens)
K562HepG2
Method: immunoblot
compliant
Caption
0.7ug unmodified Recombinant Histone H3 (Millipore 07-436) and nuclear extract from 10ug K562 and HepG2 cells were resolved by electrophoresis on a 4-12% acrylamide gel. After separation, the samples were transferred to a nitrocellulose membrane with an Invitrogen iBlot system. Membrane was blocked for an hour in room temperature, with 5% nonfat dry milk and blotted with primary antibody in the appropriate concentration over night at 4 C. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. Panel A: anti Histone H3 (Abcam ab1791), band of expected size (~17kDa) visualized in both unmodified recombinant histone H3 and NL, at similar intensities. Panel B: Band of expected size (~17kDa) visualized in K562 and HepG2 nuclear lysates, and cannot be detected in unmodified recombinant Histone H3.
Submitted by
Charles Epstein
Lab
Bradley Bernstein, Broad
H3K4me1 (Homo sapiens)
Method: dot blot assay
compliant
Caption
Performed dot blot analysis using a large set of modified peptides. The peptide most strongly recognized by the H3K4me1 antibody was the correct one.
Submitted by
Kathrina Onate
Lab
Bradley Bernstein, Broad