ENCAB469ASP
Antibody against Homo sapiens CHAMP1
Homo sapiens
K562
characterized to standards
- Status
- released
- Source (vendor)
- Bethyl Labs
- Product ID
- A304-217A
- Lot ID
- 1
- Characterized targets
- CHAMP1 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Aliases
- michael-snyder:AS-1295
- External resources
Characterizations
CHAMP1 (Homo sapiens)
K562
compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody A304-217A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
- Reviewer comment
- Just within the +/- 20% size limit.
- Submitted by
- Denis Salins
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- 1047_12_ZNF828_A304-217A.jpg
CHAMP1 (Homo sapiens)
K562
compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody A304-217A. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 89.099.
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- CHAMP1(ZNF828)_A304-217A).jpg
CHAMP1 (Homo sapiens)
compliant
- Caption
- IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using the antibody A304-217A, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
- Submitter comment
- Both Genecard and Biogrid reported that POGZ and CHAMP1 has interactions. http://www.genecards.org/cgi-bin/carddisp.pl?gene=CHAMP1&keywords=champ1. http://thebiogrid.org/129584/summary/homo-sapiens/champ1.html
- Reviewer comment
- Not the highest ranked factor but higher ranked TF (POGZ) is a known interactor.
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996