ENCAB468JSB

Antibody against Homo sapiens FAM120A

Homo sapiens
HepG2, K562
characterized to standards
Homo sapiens
any cell type or tissue
partially characterized
Status
released
Source (vendor)
Bethyl Labs
Product ID
A303-889A
Lot ID
1
Characterized targets
FAM120A (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Isotype
IgG
External resources

Characterizations

FAM120A (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Caption
IP-WB characterization of FAM120A specific antibody in K562 cell line . Lane 1 is 2.5% of five million K562 whole cell lysate Input, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal anti-OSSA. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
Submitted by
Balaji Sundararaman
Lab
Gene Yeo, UCSD
FAM120A (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
compliant
Caption
Western blot following shRNA against FAM120A in K562 and HepG2 whole cell lysate using FAM120A specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against FAM120A. Lanes 5-8 follow the same pattern, but in HepG2. FAM120A protein appears as the green band, Tubulin serves as a control and appears in red.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
FAM120A (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
IP-Western Blot analysis of HepG2 whole cell lysate using FAM120A specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-FAM120A antibody (lanes under 'FAM120A').
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD
FAM120A (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
IP-Western Blot analysis of K562 whole cell lysate using FAM120A specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-FAM120A antibody (lanes under 'FAM120A').
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD