ENCAB466YMY
Antibody against Homo sapiens ZNF184
Homo sapiens
K562
characterized to standards
- Status
- released
- Source (vendor)
- Bethyl Labs
- Product ID
- A303-738A
- Lot ID
- 1
- Characterized targets
- ZNF184 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Aliases
- michael-snyder:AS-1470
- External resources
Characterizations
ZNF184 (Homo sapiens)
K562
compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody A303-738A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 86.174
- Submitted by
- Denis Salins
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- 1073_12_ZNF184_A303-738A.jpg
ZNF184 (Homo sapiens)
K562
exempt from standards
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody A303-738A. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 86.174.
- Submitter comment
- We analyzed both immunoreactive bands by mass-spec and the TF is found in the band of expected size.
- Reviewer comment
- The band at expected size (A) does not contain >50% of the total signal in the lane as required by the standards. However, mass-spec analysis of both bands revealed the TF to be found in band A and no other TFs found in band B.
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- ZNF184_A303-738A.jpg
ZNF184 (Homo sapiens)
compliant
- Caption
- IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using the antibody A303-738A, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
- Submitter comment
- None of the proteins ranked above or equally ranked have been shown to be sequence-specific TFs (including HIC2).
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- ZNF184_A303-738A_final.pdf