ENCAB451DRR

Antibody against Homo sapiens CC2D1A

Homo sapiens
K562
characterized to standards
Status
released
Source (vendor)
Bethyl Labs
Product ID
A300-285A
Lot ID
1
Characterized targets
CC2D1A (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Aliases
michael-snyder:AS-1241
External resources

Characterizations

CC2D1A (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody A300-285A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 104.062
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
CC2D1A (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
compliant
Caption
IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using the antibody A300-285A, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitter comment
The higher ranked proteins MCM7 and TP53BP1 are not sequence-specific TFs.
Reviewer comment
The antibody review panel agreed on December 13, 2016 to allow MCM complex proteins to be ranked higher than TF of interest since MCM proteins are not sequence-specific binders.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
CC2D1A (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody A300-285A. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 104.062.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford