ENCAB449CFZ

Antibody against Homo sapiens H3K9me2

Homo sapiens
any cell type or tissue
characterized to standards
Status
released
Source (vendor)
Millipore
Product ID
17-681
Lot ID
NRG1583062
Characterized targets
H3K9me2 (Homo sapiens)
Host
mouse
Clonality
monoclonal
Aliases
bradley-bernstein:PchAb 660
External resources

Characterizations

H3K9me2 (Homo sapiens)
Method: dot blot assay
Attachment from submitter
compliant
Caption
Reactivity of test antibody with control peptides and singly modified peptides. Graph is scaled so that the intended primary reactivity is set to 1. Cross inhibition of reactivity by another modifications of the same peptide. Graph is scaled so that saturation of signal=1.
Submitted by
Marcus Ho
Lab
Bradley Bernstein, Broad
H3K9me2 (Homo sapiens)
HepG2
Method: immunoblot
compliant
Caption
Protein Load: Panel A 1 - Recombinant H3 0.7ug Panel B 1 HepG2 nuclear lysate 10ug 2 Recombinant H3 0.5ug Gel and Blot Conditions: 4-12% Bis-Tris, 1X MES running buffer iBlot transfer, nitrocellulose, P3 for 6 minutes, Blocked in TBS-T w/5% BSA for 1 hour, Antibodies diluted in TBS-T w/5% BSA 1.5 second exposure in ChemiDoc.
Submitted by
Marcus Ho
Lab
Bradley Bernstein, Broad