ENCAB445WWM

Antibody against Homo sapiens HNRNPC

Homo sapiens
HepG2, K562
not characterized to standards
Status
released
Source (vendor)
Santa Cruz Biotech
Product ID
sc-10037
Lot ID
E0912
Characterized targets
HNRNPC (Homo sapiens)
Host
goat
Clonality
polyclonal
Isotype
IgG
Antigen description
N-terminus of of hnRNP C1/C2 (HNRNPC) of human origin
Aliases
xiang-dong-fu:hnRNPC
External resources

Characterizations

HNRNPC (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
not compliant
Caption
Western blot following shRNA against HNRNPC in K562 whole cell lysate using HNRNPC specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against HNRNPC.HNRNPC protein appears as the green band, Tubulin serves as a control and appears in red.
Reviewer comment
KCO: This characterization has been deemed non-compliant because neither lane 3 nor 4 knock down the target of interest by ≥50%.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
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HNRNPC (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
IP-Western Blot analysis of HepG2 whole cell lysate using HNRNPC specific antibody. Lane 1 is 2% of ten million whole cell lysate input (lane under '10% input') , lane 2 is 20% of IP enrichment using goat normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using goat polyclonal anti-HNRNPC antibody (lanes under 'anti-HNRNPC'). Asterisk indicates heavy and light chains of antibody.
Submitted by
Marcus Ho
Lab
Xiang-Dong Fu, UCSD
HNRNPC (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
IP-Western Blot analysis of K562 whole cell lysate using HNRNPC specific antibody. Lane 1 is 2% of ten million whole cell lysate input (lane under '10% input') , lane 2 is 20% of IP enrichment using goat normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using goat polyclonal anti-HNRNPC antibody (lanes under 'anti-HNRNPC'). Asterisk indicates heavy chain of antibody.
Submitted by
Marcus Ho
Lab
Xiang-Dong Fu, UCSD
Download