ENCAB445KMF
Antibody against Homo sapiens KMT2B
Homo sapiens
K562, HepG2, GM12878
partially characterized
Homo sapiens
endothelial cell of umbilical vein, A549, H1
not characterized to standards
- Status
- released
- Source (vendor)
- Bethyl Labs
- Product ID
- A300-113A
- Lot ID
- 1
- Characterized targets
- KMT2B (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Antigen description
- a histone methyltransferase that methylates the Lys-4 position of histone H3
- Aliases
- bradley-bernstein:PchAb 1238
- External resources
Characterizations
KMT2B (Homo sapiens)
K562HepG2GM12878
compliant
- Caption
- 2mg nuclear lysates loaded for each Immunoprecipitation lane. 2 ug of primary antibody used, 50uL pA/G beads. Used 4-12% Bis-Tris gel in 1X MOPS running buffer, iBlot transfer, Program 3, 6 minutes. Blocking overnight at 4'C in TBS-T with 5% BSA. Secondary antibody added for 1 hour at RT. 2 second exposure on ChemiDoc.
- Submitted by
- Noam Shoresh
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
- Download
- MLL2_TRX2_Bethyl_A300-113A-1_IP.png
KMT2B (Homo sapiens)
A549H1
not compliant
- Caption
- Nuclear lysates from A549 (10ug) and H1 (10ug) were loaded into a 4-12% Bis-Tris gel in 1X MOPS running buffer. After separation, the samples were transferred to a nitrocellulose membrane using iblot. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. A range of bands were detected outside of the expected size.
- Reviewer comment
- KCO: Captions does not provide specific value of expected band size. Also, expected band size (stated in the image) does not correspond to >50% of all bands in its given lane (Antibody Characterizations Standards, August 2015).
- Submitted by
- Noam Shoresh
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
- Download
- MLL2_TRX2_Bethyl_A300-113A-1_V2.png
KMT2B (Homo sapiens)
K562HepG2GM12878endothelial cell of umbilical vein
not compliant
- Caption
- Nuclear lysates from K562 (10ug), HepG2 (10 ug), GM12878 (7ug), and HUVEC (10ug) were loaded into a 4-12% Bis-Tris gel in 1X MES running buffer. iBlot transfer, nitrocellulose, P3 for 8 minutes Blocked with 5% BSA in TBS-T, 1 hour at RT. Antibodies dilulted in TBS-T with 0.5% BSA. 90 second exposure in ChemiDoc.
- Reviewer comment
- KCO: Captions does not provide specific value of expected band size. Also, expected band size (stated in the image) does not correspond to >50% of all bands in its given lane (Antibody Characterizations Standards, August 2015).
- Submitted by
- Noam Shoresh
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
- Download
- MLL2_TRX2_Bethyl_A300-113A-1_WB.png