ENCAB445KMF

Antibody against Homo sapiens KMT2B

Homo sapiens
K562, GM12878, HepG2
partially characterized
Homo sapiens
A549, H1-hESC, endothelial cell of umbilical vein
not characterized to standards
Status
released
Source (vendor)
Bethyl Labs
Product ID
A300-113A
Lot ID
1
Host
rabbit
Clonality
polyclonal
Antigen description
a histone methyltransferase that methylates the Lys-4 position of histone H3
Aliases
bradley-bernstein:PchAb 1238
External resources

Characterizations

KMT2B (Homo sapiens)
K562HepG2GM12878
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
2mg nuclear lysates loaded for each Immunoprecipitation lane. 2 ug of primary antibody used, 50uL pA/G beads. Used 4-12% Bis-Tris gel in 1X MOPS running buffer, iBlot transfer, Program 3, 6 minutes. Blocking overnight at 4'C in TBS-T with 5% BSA. Secondary antibody added for 1 hour at RT. 2 second exposure on ChemiDoc.
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad
KMT2B (Homo sapiens)
A549H1-hESC
Method: immunoblot
Attachment from submitter
not compliant
Caption
Nuclear lysates from A549 (10ug) and H1 (10ug) were loaded into a 4-12% Bis-Tris gel in 1X MOPS running buffer. After separation, the samples were transferred to a nitrocellulose membrane using iblot. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. A range of bands were detected outside of the expected size.
Reviewer comment
KCO: Captions does not provide specific value of expected band size. Also, expected band size (stated in the image) does not correspond to >50% of all bands in its given lane (Antibody Characterizations Standards, August 2015).
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad
KMT2B (Homo sapiens)
K562HepG2GM12878endothelial cell of umbilical vein
Method: immunoblot
Attachment from submitter
not compliant
Caption
Nuclear lysates from K562 (10ug), HepG2 (10 ug), GM12878 (7ug), and HUVEC (10ug) were loaded into a 4-12% Bis-Tris gel in 1X MES running buffer. iBlot transfer, nitrocellulose, P3 for 8 minutes Blocked with 5% BSA in TBS-T, 1 hour at RT. Antibodies dilulted in TBS-T with 0.5% BSA. 90 second exposure in ChemiDoc.
Reviewer comment
KCO: Captions does not provide specific value of expected band size. Also, expected band size (stated in the image) does not correspond to >50% of all bands in its given lane (Antibody Characterizations Standards, August 2015).
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad