ENCAB445KMF

Antibody against Homo sapiens KMT2B

Homo sapiens
GM12878, K562, HepG2
partially characterized
Homo sapiens
A549, H1, endothelial cell of umbilical vein
not characterized to standards
Status
released
Source (vendor)
Bethyl Labs
Product ID
A300-113A
Lot ID
1
Characterized targets
KMT2B (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Antigen description
a histone methyltransferase that methylates the Lys-4 position of histone H3
Aliases
bradley-bernstein:PchAb 1238
External resources

Characterizations

KMT2B (Homo sapiens)
K562HepG2GM12878
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
2mg nuclear lysates loaded for each Immunoprecipitation lane. 2 ug of primary antibody used, 50uL pA/G beads. Used 4-12% Bis-Tris gel in 1X MOPS running buffer, iBlot transfer, Program 3, 6 minutes. Blocking overnight at 4'C in TBS-T with 5% BSA. Secondary antibody added for 1 hour at RT. 2 second exposure on ChemiDoc.
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad
KMT2B (Homo sapiens)
A549H1
Method: immunoblot
Attachment from submitter
not compliant
Caption
Nuclear lysates from A549 (10ug) and H1 (10ug) were loaded into a 4-12% Bis-Tris gel in 1X MOPS running buffer. After separation, the samples were transferred to a nitrocellulose membrane using iblot. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. A range of bands were detected outside of the expected size.
Reviewer comment
KCO: Captions does not provide specific value of expected band size. Also, expected band size (stated in the image) does not correspond to >50% of all bands in its given lane (Antibody Characterizations Standards, August 2015).
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad
KMT2B (Homo sapiens)
K562HepG2GM12878endothelial cell of umbilical vein
Method: immunoblot
Attachment from submitter
not compliant
Caption
Nuclear lysates from K562 (10ug), HepG2 (10 ug), GM12878 (7ug), and HUVEC (10ug) were loaded into a 4-12% Bis-Tris gel in 1X MES running buffer. iBlot transfer, nitrocellulose, P3 for 8 minutes Blocked with 5% BSA in TBS-T, 1 hour at RT. Antibodies dilulted in TBS-T with 0.5% BSA. 90 second exposure in ChemiDoc.
Reviewer comment
KCO: Captions does not provide specific value of expected band size. Also, expected band size (stated in the image) does not correspond to >50% of all bands in its given lane (Antibody Characterizations Standards, August 2015).
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad