ENCAB426GGE

Antibody against Aequorea victoria eGFP

Drosophila melanogaster
whole organism
partially characterized
Status
released
Source (vendor)
Abcam
Product ID
ab290
Lot ID
unknown
Characterized targets
eGFP (Aequorea victoria)
Host
rabbit
Clonality
polyclonal
Purification
crude
Antigen description
This antibody is reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP and EGFP.
External resources

Characterizations

eGFP (Aequorea victoria)
whole organism
Method: immunoblot
Attachment from submitter
compliant
Caption
Western blot with Ab290 anti-GFP (1:1000) and Licor IR680LT (Goat anti-Rabbit,1:20000). Protein from 100 mg of whole flies was extracted in 750uL T-PER (Fisher). Lane1: ladder. Lane2: 5 uL W1118 (no GFP) whole protein extract. Lane3: 5uL BDSC#5629 (nuclear GFP) whole protein extract. The band is expected at 29 kDa.
Submitted by
Alec Victorsen
Lab
Kevin White, UChicago
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eGFP (Aequorea victoria)
whole organism
Method: immunofluorescence
Attachment from submitter
compliant
Caption
Expression patterns for four transcription factors. The left column of images are the transcription expression pattern measured by in-situ hybridization on wild-type embryos as reported by the Berkeley Drosophila Genome Project (insitu.frutfly.org; PMIDs: 17645804, 12537577, 24359758). Right column are immunofluorescence patterns of GFP fused with each transcription factor. Anti-GFP (Ab290) and Alexa Fluor 488 Goat Anti-Rabbit were used for IF on the transgenic animals (ENCDO269BCO, ENCDO038HYA, ENCDO195AJO, ENCDO180UMR).
Reviewer comment
Note that according to the 2011 standards, immunofluorescence characterizations should be paired with a knockdown or knockout secondary characterization to confirm that the antibody is detecting the protein of interest.
Submitted by
Alec Victorsen
Lab
Kevin White, UChicago
Download