1. Antibodies
  2. Homo sapiens
  3. SMARCA4

ENCAB425IPH

Antibody against Homo sapiens SMARCA4

Homo sapiens
K562
characterized to standards with exemption
Status
released
Source (vendor)
Bethyl Labs
Product ID
A300-813A
Lot ID
2
Characterized targets
SMARCA4 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Aliases
michael-snyder:AS-1281
External resources

Characterizations

SMARCA4 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody A300-813A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
SMARCA4 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody A300-813A. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 184.646.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
SMARCA4 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
exempt from standards
Caption
IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using the antibody A300-813A, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitter comment
ARID1A, SMARCC1, ARID1B, SMARCC2 and SMARCA4 are components of BAF complex. http://www.genecards.org/cgi-bin/carddisp.pl?gene=SMARCA4&keywords=smarca4. ARID2 and PBRM1 have interaction with SMARCA4, http://thebiogrid.org/112481/summary/homo-sapiens/smarca4.html. ARID2 has interaction with SMARCA4, http://thebiogrid.org/112481/summary/homo-sapiens/smarca4.html. Both HNRNPUL1 (96KD) and SMARCA4 has interaction with BRD7(74KD), http://thebiogrid.org/112481/summary/homo-sapiens/smarca4.html and http://thebiogrid.org/116281/summary/homo-sapiens/hnrnpul1.html. But BRD7 is not in the gel slice with HNRNPUL1 and SMARCA4. Genecard: NONO and SFPQ form SFPQ-NONO complex, which is probably a heterotetramer of two 52 kDa (NONO) and two 100 kDa (SFPQ) subunits. http://www.genecards.org/cgi-bin/carddisp.pl?gene=NONO&keywords=nono. And NONO and SMARCA4 has interactions, 3 publications in http://thebiogrid.org/112481/summary/homo-sapiens/smarca4.html. NONO is not in the same gel slice with SFPQ and SMARCA4. ALB involves enzyme binding and chaperone binding. AZGP1 involves antigen binding and peptide antigen binding. PRPF8 is RNA-binding protein. BLMH is an enzyme.
Reviewer comment
SMARCA4 is not the top ranked TF but the submitters have noted that higher ranked TFs are part of the same BAF complex as SMARCA4.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford