ENCAB410NOJ
Alternate accession: ENCAB000AGF
Antibody against Homo sapiens SETDB1
Homo sapiens
K562, HepG2, endothelial cell of umbilical vein
partially characterized
Homo sapiens
GM12878
not characterized to standards
- Status
- released
- Source (vendor)
- Santa Cruz Biotech
- Product ID
- sc-166621
- Lot ID
- H2009
- Characterized targets
- SETDB1 (Homo sapiens)
- Host
- mouse
- Clonality
- monoclonal
- Purification
- affinity
- Isotype
- IgG2b
- Antigen description
- SET domain, bifurcated 1. Specific for an epitope mapping between amino acids 1195-1228 near the C-terminus of ESET of human origin
- Aliases
- bradley-bernstein:PchAb 359
- External resources
Characterizations
SETDB1 (Homo sapiens)
K562
Method: immunoblot
compliant
- Submitted by
- Bradley Bernstein
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG004570
SETDB1 (Homo sapiens)
Method: ChIP-string comparison
not reviewed
- Caption
- Antibody was validated by use of correlation analysis of ChIP-String data obtained using this antibody together with diverse other antibodies.
- Submitted by
- Bradley Bernstein
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG004570
SETDB1 (Homo sapiens)
K562HepG2GM12878endothelial cell of umbilical vein
Method: immunoblot
compliant
- Caption
- Nuclear lysates from K562(10ug), GM12878 (10ug),HUVEC (10ug), HepG2 (10ug), were loaded into a 4-12% Bis-Tris gel in 1X MESrunning buffer. After separation, the samples were transferred to a nitrocellulose membrane using iblot. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. two bands at expected sizes can be detected (44kDa and 150kDa).
- Submitted by
- Noam Shoresh
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991