ENCAB410NOJ

Alternate accession: ENCAB000AGF

Antibody against Homo sapiens SETDB1

Homo sapiens
at least one cell type or tissue
awaiting characterization
Homo sapiens
HepG2, endothelial cell of umbilical vein
partially characterized
Homo sapiens
GM12878, K562
not characterized to standards
Status
released
Source (vendor)
Santa Cruz Biotech
Product ID
sc-166621
Lot ID
H2009
Host
mouse
Clonality
monoclonal
Purification
affinity
Isotype
IgG2b
Antigen description
SET domain, bifurcated 1. Specific for an epitope mapping between amino acids 1195-1228 near the C-terminus of ESET of human origin
Aliases
bradley-bernstein:PchAb 359

Characterizations

SETDB1 (Homo sapiens)
Method: immunoblot
not reviewed
Submitted by
Bradley Bernstein
Lab
Bradley Bernstein, Broad
SETDB1 (Homo sapiens)
Method: ChIP-string comparison
not reviewed
Caption
Antibody was validated by use of correlation analysis of ChIP-String data obtained using this antibody together with diverse other antibodies.
Submitted by
Bradley Bernstein
Lab
Bradley Bernstein, Broad
SETDB1 (Homo sapiens)
K562HepG2GM12878endothelial cell of umbilical vein
Method: immunoblot
Attachment from submitter
compliant
Caption
Nuclear lysates from K562(10ug), GM12878 (10ug),HUVEC (10ug), HepG2 (10ug), were loaded into a 4-12% Bis-Tris gel in 1X MESrunning buffer. After separation, the samples were transferred to a nitrocellulose membrane using iblot. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. two bands at expected sizes can be detected (44kDa and 150kDa).
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad