ENCAB386GVG
Antibody against Homo sapiens HNRNPF
Homo sapiens
any cell type or tissue
partially characterized
- Status
- released
- Source (vendor)
- GeneTex
- Product ID
- GTX114476
- Lot ID
- 40135
- Characterized targets
- HNRNPF (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Isotype
- IgG
- External resources
Characterizations
HNRNPF (Homo sapiens)
Method: immunoprecipitation
not submitted for review by lab
- Caption
- IP-WB analysis of K562 whole cell lysate using HNRNPF specific antibody. Lane 1 is 2.5% of 0.5mg input lysate, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal Anti-hnRNPF antibody[N1N3]. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
- Submitted by
- Balaji Sundararaman
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- GeneTex_GTX114476_40135_HNRNPF.png
HNRNPF (Homo sapiens)
Method: knockdown or knockout
compliant
- Caption
- Western blot following CRISPR against HNRNPF in HepG2 whole cell lysate using HNRNPF specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against HNRNPF. HNRNPF protein appears as the green band, GAPDH serves as a control and appears in red.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005
- Download
- HNRNPF-HEPG2-CRISPR.png
HNRNPF (Homo sapiens)
Method: knockdown or knockout
compliant
- Caption
- Western blot following CRISPR against HNRNPF in K562 whole cell lysate using HNRNPF specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against HNRNPF. HNRNPF protein appears as the green band, GAPDH serves as a control and appears in red.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005
- Download
- HNRNPF-K562-CRISPR.png
HNRNPF (Homo sapiens)
Method: knockdown or knockout
compliant
- Caption
- Western blot following shRNA against HNRNPF in K562 and HepG2 whole cell lysate using HNRNPF specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against HNRNPF. Lanes 5-8 follow the same pattern, but in HepG2. HNRNPF protein appears as the green band, GAPDH serves as a control and appears in red.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005
- Download
- HNRNPF_Secondary_Western.png