1. Antibodies
  2. Homo sapiens
  3. ZBTB40

ENCAB373DVF

Antibody against Homo sapiens ZBTB40

Homo sapiens
K562, GM12878, MCF-7
characterized to standards
Homo sapiens
HepG2
characterized to standards with exemption
Homo sapiens
any cell type or tissue
partially characterized
Status
released
Source (vendor)
Bethyl Labs
Product ID
A301-932A
Lot ID
1
Characterized targets
ZBTB40 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Aliases
michael-snyder:AS-1095
External resources

Characterizations

ZBTB40 (Homo sapiens)
GM12878
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878, using the antibody A301-932A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 138.118
Reviewer comment
Just barely within 20% of expected size.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
ZBTB40 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
exempt from standards
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody A301-932A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 138.118
Submitter comment
TF was detected in ~100 kD band in K562 IP-MS.
Reviewer comment
Something else is more prominent near the 100kD mark relative to the marked band but the target TF was found in a band around this size in IP-MS in K562 (band A).
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
ZBTB40 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody A301-932A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 138.118
Reviewer comment
Not 50% of signal.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
ZBTB40 (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody A301-932A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 138.118
Reviewer comment
Multiple bands but marked band is >50% of total signal in lane.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
ZBTB40 (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not submitted for review by lab
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HEK293T, using the antibody A301-932A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 138.118
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
ZBTB40 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody A301-932A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 138.118
Reviewer comment
Multiple bands and band at expected size not >50% of total signal in lane.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
ZBTB40 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody A301-932A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 138.118
Reviewer comment
Multiple bands and band at expected size not >50% of total signal in lane.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
Download
 ZBTB40-K562.jpg
ZBTB40 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody A301-932A. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 138.118.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
Download
 ZBTB40.jpg
ZBTB40 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
compliant
Caption
IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using the antibody A301-932A, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitter comment
Both RIF1 and FAM208B are not DNA-binding proteins.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford