ENCAB361RPF

Antibody against Homo sapiens ZHX1

Homo sapiens
K562, HeLa-S3
characterized to standards
Homo sapiens
HepG2, GM12878
not characterized to standards
Status
released
Source (vendor)
Novus
Product ID
NB600-244
Lot ID
A1
Characterized targets
ZHX1 (Homo sapiens)
Host
rabbit
Aliases
michael-snyder:ZHX1-human_NB600-244_A1, michael-snyder:AS-154
External resources

Characterizations

ZHX1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody NB600-244. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 98.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
ZHX1 (Homo sapiens)
GM12878K562HeLa-S3HepG2
Method: immunoblot
Attachment from submitter
compliant
Caption
Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order: GM12878, K562, HeLaS3, HepG2 using the antibody NB600-244A. Molecular Weight: 98
Reviewer comment
Looks like the protein is running slightly high...
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
Download
ZHX1 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
compliant
Caption
IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using the antibody NB600-244, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford