1. Antibodies
  2. Homo sapiens
  3. EZH2

ENCAB350TQZ

Antibody against Homo sapiens EZH2

Homo sapiens
HepG2, K562, H1
characterized to standards
Homo sapiens
HCT116, SK-N-MC, PC-3, MM.1S
characterized to standards with exemption
Status
released
Source (vendor)
Cell Signaling
Product ID
5246BF
Lot ID
2
Characterized targets
EZH2 (Homo sapiens)
Host
rabbit
Clonality
monoclonal
Antigen description
enhancer of zeste homolog 2 (Drosophila)
Aliases
bradley-bernstein:PchAb 1135
External resources

Characterizations

EZH2 (Homo sapiens)
HCT116SK-N-MCPC-3MM.1S
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
exempt from standards
Caption
This is a request for "exemption from further primary validation" for an additional EZH2 antibody. It was successfully western blotted in 3 cell types, and a secondary validation was accepted. We are asking for this antibody to be valid for a total of four additional cell types not included in the western blot we supplied to DCC.
Submitter comment
This antibody has been requested for exemption.
Reviewer comment
Peggy Farnham approved this request on behalf of the antibody standards working group.
Submitted by
Charles Epstein
Lab
Bradley Bernstein, Broad
Download
 exempted.png
EZH2 (Homo sapiens)
HepG2K562H1
Method: immunoprecipitation
Attachment PDF Icon
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell lines H1-hESC (lane 2), HepG2 (lane 3), and K562 (lane 4) using the antibody ab6721 (Lot GR119224-3). The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using rabbit IgG. Target molecular weight antibody: 98kDa. https://www.cellsignal.com/products/primary-antibodies/ezh2-d2c9-xp-rabbit-mab/5246
Submitted by
Kathrina Onate
Lab
Bradley Bernstein, Broad
EZH2 (Homo sapiens)
Method: ChIP-seq comparison
Attachment PDF Icon
compliant
Caption
This validation relies on the use of antibodies to a chromatin regulator and a functionally related histone modification in MM.1S cells, and the demonstration that highly similar patterns of enrichment are obtained with each antibody. The first track shown used an antibody to phosphorylated EZH2 (PchAb 1135, ENCAB350TQZ), and the second track shown used an antibody to H3K27me3 previously characterized to Encode standards (PchAb 683, ENCAB000BLA). Overall genomewide correlation: >0.85
Submitted by
Nina Farrell
Lab
Bradley Bernstein, Broad