ENCAB349QGP

Antibody against Homo sapiens NONO

Homo sapiens
HepG2, GM12878, HEK293T, MCF-7, K562
characterized to standards
Status
released
Source (vendor)
Sigma
Product ID
N8664
Lot ID
089K4755
Characterized targets
NONO (Homo sapiens)
Host
rabbit
Aliases
michael-snyder:NONO-human_N8664_089K4755
External resources

Characterizations

NONO (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody N8664. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 55.0
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
NONO (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody N8664. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 55.0
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
NONO (Homo sapiens)
HEK293T
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HEK293T, using the antibody N8664. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 55.0
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
NONO (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
not compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody N8664. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Reviewer comment
Overloaded? Signal at expected size is a blob between the 50kD and 75kD markers with a lower sized band below.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
NONO (Homo sapiens)
GM12878
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878, using the antibody N8664. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 55.0
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
NONO (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody N8664. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 55.0
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
NONO (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody N8664. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 55.0
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
NONO (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody N8664. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 55.0.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
Download
NONO (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
compliant
Caption
IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using the antibody N8664, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitter comment
Biogrid: Nono has interaction with HNRNPA1, 3 publications; Rad50 also has interaction with HNRNPA1 (1 publication). http://thebiogrid.org/110904/summary/homo-sapiens/nono.html; http://thebiogrid.org/115417/summary/homo-sapiens/rad50.html. MRE11Aforms a complex with the RAD50 homolog, http://www.genecards.org/cgi-bin/carddisp.pl?gene=MRE11A&keywords=mre11a. Genecard: Nono: Monomer and component of the SFPQ-NONO complex, which is probably a heterotetramer of two 52 kDa (NONO) and two 100 kDa (SFPQ) subunits. http://www.genecards.org/cgi-bin/carddisp.pl?gene=NONO&keywords=nono.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford