1. Antibodies
  2. Homo sapiens
  3. CBX5

ENCAB329LKX

Antibody against Homo sapiens CBX5

Homo sapiens
K562, HepG2, GM12878, SK-N-SH
characterized to standards
Homo sapiens
H1
characterized to standards with exemption
Status
released
Source (vendor)
Millipore
Product ID
05-689
Lot ID
DAM1810805
Characterized targets
CBX5 (Homo sapiens)
Host
mouse
Clonality
monoclonal
Antigen description
chromobox homolog 5; heterochromatin protein 1-alpha
Aliases
bradley-bernstein:PchAb 530
External resources

Characterizations

CBX5 (Homo sapiens)
Method: ChIP-seq comparison
Attachment PDF Icon
compliant
Caption
This validation relies on the use of antibodies to a chromatin regulator (CBX5) and a histone modification (H3K9me3) in H1 cells, and the demonstration that highly similar patterns of enrichment are obtained with each antibody. The first track shown used an antibody to CBX5 (PchAb 530, ENCAB329LKX), and the second track shown used an antibody to H3K9me3 previously characterized to Encode standards (PchAb 125-V, ENCAB000APY).
Submitter comment
SAV4
Submitted by
Nina Farrell
Lab
Bradley Bernstein, Broad
CBX5 (Homo sapiens)
K562HepG2GM12878SK-N-SHH1
Method: immunoblot
Attachment from submitter
Attachment from submitters
compliant
Caption
Nuclear lysates from K562 (10ug), HepG2 (7ug), GM12878 (7ug), SK-N-SH (6ug), were loaded into a 4-12% Bis-Tris gel in 1X MOPS running buffer. After separation, the samples were transferred to a nitrocellulose membrane with an Invitrogen iBlot system. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. a single band of the expected size was detected (~29kDa).
Submitter comment
The antibody review board exempted Hi-hESC data from primary characterization requirement based on the quality of the ChIP of the two replicates and the other cell lines 03-05-2018
Reviewer comment
From Peggy: For this CBX5 antibody (ENCAB329LKX), I would be supportive of releasing experiment ENCSR814KIO since it pass characterization standards in other cell lines.
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad