1. Antibodies
  2. Homo sapiens
  3. NR2C1

ENCAB324ARN

Antibody against Homo sapiens NR2C1

Homo sapiens
K562, MCF-7, GM12878, HepG2
characterized to standards with exemption
Status
released
Source (vendor)
Bethyl Labs
Product ID
A303-047A
Lot ID
1
Characterized targets
NR2C1 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Aliases
michael-snyder:AS-1275
External resources

Characterizations

NR2C1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
exempt from standards
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody A303-047A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG. Expected Molecular Weight 67 KD
Submitter comment
-
Reviewer comment
Since both bands disappear with IgG, I think they both are the protein
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
NR2C1 (Homo sapiens)
GM12878
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
exempt from standards
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878 using the antibody A303-047A. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 67.315.
Submitter comment
1) same pattern as K562 IP. 2) Mass Spec result indicates that the both bands (~50-75kd) are NR2C1
Reviewer comment
Marked band at expected size not 50% of total signal in lane is consistent with banding pattern in K562 which was analyzed by mass spec and confirmed to detect NR2C1.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
NR2C1 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
exempt from standards
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2 using the antibody A303-047A. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 67.315.
Submitter comment
1) same pattern as K562 IP. 2) Mass Spec result indicates that the both bands (~50-75kd) are NR2C1
Reviewer comment
Marked band at expected size not 50% of total signal in lane is consistent with banding pattern in K562 which was analyzed by mass spec and confirmed to detect NR2C1.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
NR2C1 (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
exempt from standards
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7 using the antibody A303-047A. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 67.315.
Submitter comment
1) same pattern as K562 IP. 2) Mass Spec result indicates that the both bands (~50-75kd) are NR2C1
Reviewer comment
Marked band at expected size not 50% of total signal in lane is consistent with banding pattern in K562 which was analyzed by mass spec and confirmed to detect NR2C1.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
NR2C1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
exempt from standards
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody A303-047A. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 67.315.
Submitter comment
-
Reviewer comment
Cut section analyzed by mass-spec encompasses two immunoreactive bands analyzed by mass-spec, but banding pattern is consistent with vendor's IPs. Mass-spec detected NR2C1
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
NR2C1 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
compliant
Caption
IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using the antibody A303-047A, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitter comment
CCAR1, FUS and DLAT are not sequence-specific DNA binding TFs.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford