ENCAB323UEU
Antibody against Homo sapiens H3K27me3
Homo sapiens
any cell type or tissue
characterized to standards
- Status
- released
- Source (vendor)
- Diagenode
- Product ID
- pAb-195-050
- Lot ID
- A1811-001P
- Characterized targets
- H3K27me3 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Aliases
- bradley-bernstein:PchAb 727
- External resources
Characterizations
H3K27me3 (Homo sapiens)
HeLa-S3
compliant
- Caption
- We performed a WB using 0.7ug unmodified recombinant Histone H3 (Millipore 14-494) and 20ug of HeLa Nuclear Lysate. Lysates were resolved by electrophoresis on a 4-12% bis-tris gel. After separation, the samples were transferred to a nitrocellulose membrane with an Invitrogen iBlot system. Membrane was blocked for an hour in room temperature, with 5% nonfat dry milk and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. Band of expected size (~17kDa) visualized in HeLa nuclear extract and cannot be detected in unmodified recombinant Histone H3. Antibody seems to be specific since no reactivity with non histone proteins or unmodified histones was detected.
- Submitted by
- Nina Farrell
- Lab
- Bradley Bernstein, Broad
- Grant
- UM1HG009390
H3K27me3 (Homo sapiens)
compliant
- Caption
- Performed dot blot analysis using a large set of modified peptides. The only peptide recognized by the H3K27me3 antibody was the correct one.
- Submitted by
- Nina Farrell
- Lab
- Bradley Bernstein, Broad
- Grant
- UM1HG009390