ENCAB315HBO

Antibody against Homo sapiens CREBBP

Homo sapiens
K562, HepG2, GM12878, endothelial cell of umbilical vein
partially characterized
Status
released
Source (vendor)
Cell Signaling
Product ID
7389BF
Lot ID
2
Characterized targets
CREBBP (Homo sapiens)
Host
rabbit
Clonality
monoclonal
Antigen description
cAMP-response element binding protein binding protein
Aliases
bradley-bernstein:PchAb 1252
External resources

Characterizations

CREBBP (Homo sapiens)
K562HepG2GM12878endothelial cell of umbilical vein
Method: immunoblot
Attachment from submitter
compliant
Caption
Nuclear lysates from K562 (10ug), HepG2 (10ug), GM12878 (10ug), HUVEC (12ug), were loaded into a 3-8% TA gel in 1X TA running buffer. After separation, the samples were transferred to a nitrocellulose membrane using a wet transfer. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min.the strongest band was detected around the expected size (~260kDa).
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad