ENCAB315HBO
Antibody against Homo sapiens CREBBP
Homo sapiens
K562, HepG2, GM12878, endothelial cell of umbilical vein
partially characterized
- Status
- released
- Source (vendor)
- Cell Signaling
- Product ID
- 7389BF
- Lot ID
- 2
- Characterized targets
- CREBBP (Homo sapiens)
- Host
- rabbit
- Clonality
- monoclonal
- Antigen description
- cAMP-response element binding protein binding protein
- Aliases
- bradley-bernstein:PchAb 1252
- External resources
Characterizations
CREBBP (Homo sapiens)
K562HepG2GM12878endothelial cell of umbilical vein
compliant
- Caption
- Nuclear lysates from K562 (10ug), HepG2 (10ug), GM12878 (10ug), HUVEC (12ug), were loaded into a 3-8% TA gel in 1X TA running buffer. After separation, the samples were transferred to a nitrocellulose membrane using a wet transfer. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min.the strongest band was detected around the expected size (~260kDa).
- Submitted by
- Noam Shoresh
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991