ENCAB315FWL
Antibody against Homo sapiens DDX21
Homo sapiens
HepG2, K562
characterized to standards
Homo sapiens
any cell type or tissue
partially characterized
- Status
- released
- Source (vendor)
- MBLI
- Product ID
- RN090PW
- Lot ID
- 001
- Characterized targets
- DDX21 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Antigen description
- Peptide, C-terminus of human DDX21
- External resources
Characterizations
DDX21 (Homo sapiens)
Method: knockdown or knockout
compliant
- Caption
- Western blot following CRISPR against DDX21 in K562 whole cell lysate using DDX21 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DDX21. DDX21 protein appears as the green band, Tubulin serves as a control and appears in red.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005
- Download
- DDX21-K562-CRISPR.png
DDX21 (Homo sapiens)
Method: knockdown or knockout
compliant
- Caption
- Western blot following shRNA against DDX21 in K562 and HepG2 whole cell lysate using DDX21 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against DDX21. Lanes 5-8 follow the same pattern, but in HepG2. DDX21 protein appears as the green band, Tubulin serves as a control and appears in red.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005
- Download
- DDX21_Secondary_Western.png
DDX21 (Homo sapiens)
HepG2
Method: immunoprecipitation
compliant
- Caption
- IP-Western Blot analysis of HepG2 whole cell lysate using DDX21 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-DDX21 antibody (lanes under 'DDX21').
- Submitted by
- Steven Blue
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- HepG2_MBLI_RN090PW_001_DDX21.png
DDX21 (Homo sapiens)
K562
Method: immunoprecipitation
compliant
- Caption
- IP-Western Blot analysis of K562 whole cell lysate using DDX21 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-DDX21 antibody (lanes under 'DDX21').
- Submitted by
- Balaji Sundararaman
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- K5622_MBLI_RN090PW_001_DDX21.png
DDX21 (Homo sapiens)
Method: immunoprecipitation
not submitted for review by lab
- Caption
- IP-WB analysis of K562 whole cell lysate using DDX21 specific antibody. Lane 1 is 2.5% of 0.5mg input lysate, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal Anti-DDX21pAb. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
- Submitted by
- Balaji Sundararaman
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- MBLI_RN090PW_001_DDX21.png