1. Antibodies
  2. Homo sapiens
  3. SMARCC2

ENCAB313DWJ

Antibody against Homo sapiens SMARCC2

Homo sapiens
K562, HepG2, MCF-7
characterized to standards with exemption
Status
released
Source (vendor)
Bethyl Labs
Product ID
A301-039A
Lot ID
1
Characterized targets
SMARCC2 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Aliases
michael-snyder:AS-1286
External resources

Characterizations

SMARCC2 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody A301-039A. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 132.879.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
SMARCC2 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody A301-039A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Reviewer comment
+20% allows up to 159.6kD size be tolerated.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
SMARCC2 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2 using the antibody A301-039A. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 132.879.
Reviewer comment
Marked band is higher than expected size but within allowed 20% deviation and is consistent with pattern in other cell lines.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
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 3.jpg
SMARCC2 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
exempt from standards
Caption
IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using the antibody A301-039A, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitter comment
ARID1A, SMARCA4, ARID1B, SMARCC1, SMARCA2 and SMARCC2 are component of the BAF complex, http://www.genecards.org/cgi-bin/carddisp.pl?gene=SMARCC2&keywords=smarcc2. Both HNRNPUL1 (96KD) and SMARCC2 have interaction with BRD7(74KD), http://thebiogrid.org/112485/summary/homo-sapiens/smarcc2.html and http://thebiogrid.org/116281/summary/homo-sapiens/hnrnpul1.html. But BRD7 is not in the gel slice with HNRNPUL1 and SMARCC2. DSP involves poly(A) RNA binding and structural constituent of cytoskeleton. NUP210 is not a DNA binding protein. ARID2 and SMARCC2 have interaction, 3 publications on http://thebiogrid.org/112485/summary/homo-sapiens/smarcc2.html. Both JUP and SMARCCA2 has interaction with EED (50KD), http://thebiogrid.org/112485/summary/homo-sapiens/smarcc2.html and http://thebiogrid.org/109931/summary/homo-sapiens/jup.html. PBRM1 and EWSR1 have interactions with SMARCC2, http://thebiogrid.org/112485/summary/homo-sapiens/smarcc2.html. ALB involves enzyme binding and chaperone binding. AZGP1 involves antigen binding and peptide antigen binding. ANXA2 involves poly(A) RNA binding and Rab GTPase binding.
Reviewer comment
Although SMARCC2 is not the top ranked TF, higher ranked TFs are part of the BAF complex with SMARCC2 as argued by the submitters (PMID: 20550931).
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
SMARCC2 (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7 using the antibody A301-039A. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 132.879.
Reviewer comment
Marked band is higher than expected size but within allowed 20% deviation and is consistent with pattern in other cell lines.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford