ENCAB306IYD
Antibody against Homo sapiens ATF4
Homo sapiens
K562, HepG2, HEK293T
partially characterized
Homo sapiens
at least one cell type or tissue
not pursued
Homo sapiens
MCF-7
not characterized to standards
- Status
- released
- Source (vendor)
- Cell Signaling
- Product ID
- 11815S
- Lot ID
- 2
- Characterized targets
- ATF4 (Homo sapiens)
- Host
- rabbit
- Clonality
- monoclonal
- Isotype
- IgG
- Antigen description
- Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ATF-4 protein.
- Aliases
- michael-snyder:AS-1184
- External resources
Characterizations
ATF4 (Homo sapiens)
K562
Method: immunoprecipitation
compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody 11815S. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
- Submitted by
- Denis Salins
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- 1048_1-ATF4_11815S.jpg
ATF4 (Homo sapiens)
MCF-7
Method: immunoprecipitation
not compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody 11815S. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 38.590
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- 1109_01_ATF4_11815S.jpg
ATF4 (Homo sapiens)
HepG2
Method: immunoprecipitation
compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody 11815S. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 38.590
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- 1109_02_ATF4_11815S.jpg
ATF4 (Homo sapiens)
HEK293T
Method: immunoprecipitation
compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: HEK293T, using the antibody 11815S. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 38.590
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- 1112_02_ATF4_11815S.jpg
ATF4 (Homo sapiens)
K562
Method: immunoprecipitation
not submitted for review by lab
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody 11815S. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 38.590.
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- ATF4.jpg
ATF4 (Homo sapiens)
Method: immunoprecipitation
not submitted for review by lab
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878, using the antibody 11815S. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 38.590
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- expt1110_1-ATF4-11815S.jpg