1. Antibodies
  2. Homo sapiens
  3. ZBTB5

ENCAB301QZF

Antibody against Homo sapiens ZBTB5

Homo sapiens
K562
characterized to standards with exemption
Status
released
Source (vendor)
Bethyl Labs
Product ID
A304-063A
Lot ID
1
Characterized targets
ZBTB5 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Aliases
michael-snyder:AS-1402
External resources

Characterizations

ZBTB5 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody A304-063A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 74.278
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
ZBTB5 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody A304-063A. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 74.278.
Reviewer comment
There are multiple immunoreactive bands but the marked band has >= 50% of the total signal in the lane as required by the standards.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
ZBTB5 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
exempt from standards
Caption
IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using the antibody A304-063A, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitter comment
None of the proteins ranked above or equally ranked have been shown to be sequence-specific TFs (including ILF3, TR150, FUS, PNN, ADAR, TAF15, DDX1, TOP1).
Reviewer comment
Not all immunoreactive bands were analyzed by mass-spec. Obviously, it's too late fix that now and only what was submitted can be evaluated. The targeted TF can be detected
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford