1. Antibodies
  2. Homo sapiens
  3. FOXA1

ENCAB301QLE

Antibody against Homo sapiens FOXA1

Homo sapiens
MCF-7, HepG2, K562, HEK293T
characterized to standards
Status
released
Source (vendor)
GeneTex
Product ID
GTX100308
Lot ID
39435
Characterized targets
FOXA1 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Aliases
michael-snyder:AS-1210
External resources

Characterizations

FOXA1 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation of FOXA1 from HepG2 cells using GTX100308. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with GTX100308. Lane 3: material immunoprecipitated using control IgG. Band A was excised from gel and subject to analysis by mass spectrometry. The expected band size is 49 kDa.
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
Download
 1005_FOXA1.jpg
FOXA1 (Homo sapiens)
Method: motif enrichment
Attachment PDF Icon
compliant
Caption
The motif for target FOXA1 is represented by the attached position weight matrix (PWM) derived from ENCFF507INR. Motif enrichment analysis was done by Dr. Zhizhuo Zhang (Broad Institute, Kellis Lab). Accept probability score: 0.920250256 Global enrichment Z-score: 7.761666006 Positional bias Z-score: 10.05333536 Peak rank bias Z-score: 6.646688305 Enrichment rank: 1.0
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
FOXA1 (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody GTX100308. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
FOXA1 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody GTX100308. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 49.148
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
FOXA1 (Homo sapiens)
HEK293T
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HEK293T, using the antibody GTX100308. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 49.148
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
FOXA1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody GTX100308. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 49.148
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
FOXA1 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
compliant
Caption
IP followed by mass spectrometry: Briefly, protein was immunoprecipitated from HepG2 nuclear cell lysates using GTX100308, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitter comment
For this factor, we found another TF called NONO has more peptides than targeted protein FOXA1. We would like to explain as follows: FOXA1 has interaction with AR, http://thebiogrid.org/109411/summary/homo-sapiens/foxa1.html And NONO also has interaction with AR http://thebiogrid.org/110904/summary/homo-sapiens/nono.html
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford