ENCAB298WVV
Antibody against Homo sapiens U2AF1
Homo sapiens
K562, HepG2
characterized to standards
- Status
- released
- Source (vendor)
- Bethyl Labs
- Product ID
- A302-079A
- Lot ID
- 1
- Characterized targets
- U2AF1 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Isotype
- IgG
- Aliases
- xiang-dong-fu:U2AF1
- External resources
Characterizations
U2AF1 (Homo sapiens)
HepG2
Method: immunoprecipitation
compliant
- Caption
- IP-Western Blot analysis of HepG2 whole cell lysate using U2AF1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-U2AF1 antibody (lanes under 'U2AF1').
- Submitted by
- Balaji Sundararaman
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- HepG2_Bethyl_A302-079A_1_U2AF1.png
U2AF1 (Homo sapiens)
K562
Method: immunoprecipitation
compliant
- Caption
- IP-Western Blot analysis of K562 whole cell lysate using U2AF1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-U2AF1 antibody (lanes under 'U2AF1').
- Submitted by
- Balaji Sundararaman
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- K562_Bethyl_A302-079A_1_U2AF1.png
U2AF1 (Homo sapiens)
Method: knockdown or knockout
compliant
- Caption
- Western blot following shRNA against U2AF1 in HepG2 whole cell lysate using U2AF1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against U2AF1.U2AF1 protein appears as the green band, Tubulin serves as a control and appears in red.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005
- Download
- U2AF1-HEPG2_Secondary_Western.png
U2AF1 (Homo sapiens)
Method: knockdown or knockout
compliant
- Caption
- Western blot following shRNA against U2AF1 in K562 whole cell lysate using U2AF1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against U2AF1.U2AF1 protein appears as the green band, Tubulin serves as a control and appears in red.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005
- Download
- U2AF1-k562_Secondary_Western.png