ENCAB297SNP

Antibody against Homo sapiens PPP1R10

Homo sapiens
K562, MCF-7, HepG2, HEK293T
characterized to standards
Homo sapiens
any cell type or tissue
partially characterized
Status
released
Source (vendor)
Bethyl Labs
Product ID
A300-440A
Lot ID
1
Characterized targets
PPP1R10 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Aliases
michael-snyder:AS-1559
External resources

Characterizations

PPP1R10 (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody A300-440A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 99.058
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
PPP1R10 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody A300-440A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 99.058
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
PPP1R10 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody A300-440A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 99.058
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
PPP1R10 (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878, using the antibody A300-440A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 99.058
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
PPP1R10 (Homo sapiens)
HEK293T
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HEK293T, using the antibody A300-440A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 99.058
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
PPP1R10 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line HepG2 using the antibody A300-440A. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 99.058.
Reviewer comment
Compared to the other submitted IP in HepG2 and in other cell types, the immunoreactive band here looks to be the smaller sized secondary band seen in the other IPs. However, it is within 20% of the allowed size deviation and subsequent mass-spec analysis revealed the TF to be detected in this band.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
PPP1R10 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
compliant
Caption
IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from HepG2 nuclear cell lysates using the antibody A300-440A, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitter comment
None of the proteins ranked above or equally ranked have been shown to be sequence-specific TFs (including ILF3, EWS, FUS).
Reviewer comment
The analyzed band looks to be the smaller sized (and smaller than expected size) of two bands detected in the other IPs. It's conceivable the targeted TF (or other TFs) can also be found in the band at expected size but that band was not detected in the analyzed IP.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford