ENCAB297EGB

Antibody against Homo sapiens DDX1

Homo sapiens
any cell type or tissue
partially characterized
Status
released
Source (vendor)
GeneTex
Product ID
GTX105205
Lot ID
39904
Characterized targets
DDX1 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Isotype
IgG
External resources

Characterizations

DDX1 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
compliant
Caption
Western blot following shRNA against DDX1 in K562 and HepG2 whole cell lysate using DDX1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against DDX1. Lanes 5-8 follow the same pattern, but in HepG2. DDX1 protein appears as the green band, Tubulin serves as a control and appears in red.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
DDX1 (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Caption
IP-WB characterization of DDX1 specific antibody in K562 cell line . Lane 1 is 2.5% of five million K562 whole cell lysate Input, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal Anti-DDX1[N3C2]. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
Submitted by
Balaji Sundararaman
Lab
Gene Yeo, UCSD