ENCAB284NOX

Antibody against Homo sapiens GEMIN2

Homo sapiens
any cell type or tissue
partially characterized
Homo sapiens
K562
not characterized to standards
Status
released
Source (vendor)
MBLI
Product ID
RN102PW
Lot ID
001
Characterized targets
GEMIN2 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
External resources

Characterizations

GEMIN2 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
compliant
Caption
Western blot following CRISPR against GEMIN2 in HepG2 whole cell lysate using GEMIN2 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against GEMIN2. GEMIN2 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
GEMIN2 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
compliant
Caption
Western blot following CRISPR against GEMIN2 in K562 whole cell lysate using GEMIN2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against GEMIN2. GEMIN2 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
GEMIN2 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
not compliant
Caption
IP-WB analysis of K562 whole cell lysate using the GEMIN2 specific antibody, RN102PW. Lane 1 is 2.5% of five million whole cell lysate input. Lanes 2 and 3 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the GEMIN2-specific antibody, RN102PW. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
Reviewer comment
Lane changed to 3 (2022/08/08).
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD
GEMIN2 (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Caption
IP-WB analysis of K562 whole cell lysate using GEMIN2 specific antibody. Lane 1 is 2.5% of five million whole cell lysate Input, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal Anti-GEMIN2 (Human) pAb. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
Submitted by
Balaji Sundararaman
Lab
Gene Yeo, UCSD