ENCAB279BHV

Antibody against Homo sapiens CREB1

Homo sapiens
K562, MCF-7, GM12878, HepG2, HEK293T
characterized to standards
Status
released
Source (vendor)
Cell Signaling
Product ID
9197S
Lot ID
16
Characterized targets
CREB1 (Homo sapiens)
Host
rabbit
Clonality
monoclonal
Aliases
michael-snyder:AS-1186
External resources

Characterizations

CREB1 (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
not compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody 9197S. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Reviewer comment
Marked band not in input and is smeared in IP making it hard to distinguish if signal is there that isn't in the IgG.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
CREB1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody 9197S. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 36.688
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
CREB1 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody 9197S. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 36.688
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
CREB1 (Homo sapiens)
HEK293T
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HEK293T, using the antibody 9197S. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 36.688
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
CREB1 (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody 9197S. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 36.688
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
CREB1 (Homo sapiens)
Method: motif enrichment
compliant
Caption
The motif for target CREB1 is represented by the attached position weight matrix (PWM) derived from ENCFF336QYE. Motif enrichment analysis was done by Dr. Zhizhuo Zhang (Broad Institute, Kellis Lab) using known motifs (http://compbio.mit.edu/encode-motifs/) and previously published ChIP-seq data (http://www.broadinstitute.org/~zzhang/motifpipeline/data/TrainSetInfo.txt). The accept probability score of the given transcription factor was calculated using a Bayesian approach. This analysis also includes three motif enrichment scores, computed by overlapping the motif instances with the given ChIP-seq peak locations, as well as an enrichment rank. For more information on the underlying statistical methods, please see the attached document. Accept probability score: 0.739970154 Global enrichment Z-score: 8.795339264 Positional bias Z-score: 13.14656578 Peak rank bias Z-score: 10.07841507 Enrichment rank: 1.0
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
CREB1 (Homo sapiens)
GM12878
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878, using the antibody 9197S. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 36.688
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford