1. Antibodies
  2. Homo sapiens
  3. CHD4

ENCAB276UJU

Antibody against Homo sapiens CHD4

Homo sapiens
K562, HepG2, GM12878, endothelial cell of umbilical vein
characterized to standards
Homo sapiens
A549
characterized to standards with exemption
Status
released
Source (vendor)
Bethyl Labs
Product ID
A301-081A
Lot ID
3
Characterized targets
CHD4 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Antigen description
helicase component of an HDAC complex; repressive
Aliases
bradley-bernstein:PchAb 1222
External resources

Characterizations

CHD4 (Homo sapiens)
Method: ChIP-seq comparison
Attachment PDF Icon
compliant
Caption
This validation relies on the use of antibodies to different members of a known complex, in HepG2 cells, and the demonstration that highly similar patterns of enrichment are obtained with each antibody. The first and third tracks shown both used an antibody to CHD4 (PchAb 1222), and the second and fourth tracks shown used antibodies to HDAC2 (PchAb 317) and KDM1A (Pch 158), other members of the NuRD complex. Overall correlation scores: CHD4 vs. HDAC2: 0.8539; CHD4 vs. KDM1A: 0.8585
Submitted by
Kathrina Onate
Lab
Bradley Bernstein, Broad
CHD4 (Homo sapiens)
K562HepG2GM12878endothelial cell of umbilical veinA549
Method: immunoblot
Attachment from submitter
Attachment from submitters
compliant
Caption
Nuclear lysates from K562 (10ug), HepG2 (10ug), GM12878 (7ug), HUVEC (10ug), were loaded into a 4-12% Bis-Tris gel in 1X MOPS running buffer. After separation, the samples were transferred to a PVDF membrane using wet transfer. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. a single band at the expected size (~270kDa) was detected.
Submitter comment
The antibody review board exempted A549 data from primary characterization requirement based on the quality of the ChIP of the two replicates and the other cell lines 03-05-2018
Reviewer comment
I would be supportive of releasing this since the antibody passes characterization standards in other cell lines.
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad