ENCAB275OHR
Antibody against Homo sapiens HNRNPC
Homo sapiens
K562, HepG2
characterized to standards
Homo sapiens
any cell type or tissue
partially characterized
- Status
- released
- Source (vendor)
- MBLI
- Product ID
- RN052PW
- Lot ID
- 001
- Characterized targets
- HNRNPC (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Antigen description
- Peptide, internal region of human HNRNPC
- External resources
Characterizations
HNRNPC (Homo sapiens)
HepG2
Method: immunoprecipitation
compliant
- Caption
- IP-Western Blot analysis of HepG2 whole cell lysate using HNRNPC specific antibody. Lane 1 is 1% of twenty million whole cell lysate input, lane 2 is 1% supernatant after immunoprecipitation and lane 3 is 10% of IP enrichment using rabbit polyclonal anti-HNRNPC antibody (lanes under 'HNRNPC'). Lane 4 is 1% of twenty million whole cell lysate input and lane 5 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG').
- Submitted by
- Balaji Sundararaman
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- HepG2_MBLI_RN052PW_001_HNRNPC.png
HNRNPC (Homo sapiens)
Method: knockdown or knockout
compliant
- Caption
- Western blot following shRNA against HNRNPC in K562 and HepG2 whole cell lysate using HNRNPC specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against HNRNPC. Lanes 5-7 follow the same pattern, but in HepG2. HNRNPC protein appears as the green band, GAPDH serves as a control and appears in red.
- Submitted by
- Brenton Graveley
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005
- Download
- HNRNPC_Secondary_Western.png
HNRNPC (Homo sapiens)
Method: immunoprecipitation
not submitted for review by lab
- Caption
- IP-WB characterization of HNRNPC specific antibody in K562 cell line . Lane 1 is 2.5% of five million K562 whole cell lysate Input, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal Anti-HNRNPCpAb. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
- Submitted by
- Balaji Sundararaman
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- MBLI_RN052PW_001_HNRNPC_K562.png
HNRNPC (Homo sapiens)
K562
Method: immunoprecipitation
compliant
- Caption
- IP-WB characterization of HNRNPC specific antibody in K562 cell line . Lane 1 is 2.5% of five million K562 whole cell lysate Input, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal Anti-HNRNPCpAb. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
- Submitted by
- Balaji Sundararaman
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- MBLI_RN052PW_001_HNRNPC_K562.png
HNRNPC (Homo sapiens)
Method: immunoprecipitation
not submitted for review by lab
- Caption
- IP-WB characterization of HNRNPC specific antibody in MCF7 cell line . Lane 1 is 2.5% of 0.5mg MCF7 whole cell lysate Input, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal Anti-HNRNPCpAb. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
- Submitted by
- Balaji Sundararaman
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- MBLI_RN052PW_001_HNRNPC_MCF7.png