ENCAB260LTI

Antibody against Homo sapiens E2F7

Homo sapiens
K562
characterized to standards
Status
released
Source (vendor)
Bethyl Labs
Product ID
A303-037A
Lot ID
1
Characterized targets
E2F7 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Aliases
michael-snyder:AS-1541
External resources

Characterizations

E2F7 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562 using the antibody A303-037A. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 99.888.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
E2F7 (Homo sapiens)
Method: motif enrichment
compliant
Caption
The motif for target E2F7 is represented by the attached position weight matrix (PWM) derived from ENCFF310AGG. Motif enrichment analysis was done by Dr. Zhizhuo Zhang (Broad Institute, Kellis Lab) using known motifs (http://compbio.mit.edu/encode-motifs/) and previously published ChIP-seq data (http://www.broadinstitute.org/~zzhang/motifpipeline/data/TrainSetInfo.txt). The accept probability score of the given transcription factor was calculated using a Bayesian approach. This analysis also includes three motif enrichment scores, computed by overlapping the motif instances with the given ChIP-seq peak locations, as well as an enrichment rank. For more information on the underlying statistical methods, please see the attached document. Accept probability score: 0.895771186972 Global enrichment Z-score: 5.23624196162 Positional bias Z-score: 4.16536470444 Peak rank bias Z-score: 6.3202097382 4 Enrichment rank: 1.0
Submitted by
Jessika Adrian
Lab
Michael Snyder, Stanford