1. Antibodies
  2. Homo sapiens
  3. ZNF407

ENCAB222KVZ

Antibody against Homo sapiens ZNF407

Homo sapiens
K562
characterized to standards
Status
released
Source (vendor)
Bethyl Labs
Product ID
A304-181A
Lot ID
1
Characterized targets
ZNF407 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Aliases
michael-snyder:AS-1459
External resources

Characterizations

ZNF407 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody A304-181A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 247.367
Reviewer comment
Major band corresponds to <50% of the total signal in the lane
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
ZNF407 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody A304-181A. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 247.367.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
ZNF407 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
compliant
Caption
IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using the antibody A304-181A, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitter comment
DDX21, SMARCA5, TOP2A, HNRNPUL1, AHCTF1, ILF3 were also detected in MS of ZNF407 (ENCAB019LBH). MKI167 involves poly(A) RNA binding and protein C-terminus binding. KIF21A is a microtubule-binding motor protein.
Reviewer comment
Peptides ranked higher than ZNF407 were also detected in an independent mass-spec analysis using a different antibody against ZNF407 (ENCAB019LBH).
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford