1. Antibodies
  2. Homo sapiens
  3. ATM

ENCAB219NWE

Antibody against Homo sapiens ATM

Homo sapiens
HepG2
characterized to standards
Status
released
Source (vendor)
Bethyl Labs
Product ID
A300-135A
Lot ID
1
Characterized targets
ATM (Homo sapiens)
Host
goat
Clonality
polyclonal
Purification
affinity
Aliases
michael-snyder:AS-856
External resources

Characterizations

ATM (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
compliant
Caption
IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from HepG2 nuclear cell lysates using the antibody A300-135A, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
ATM (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
exempt from standards
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody A300-135A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Submitter comment
Sorry, we didn't have a better ladder.
Reviewer comment
Band is very small, is there a bigger ladder that can be used? It's tough evaluate whether it's within 20% of expected size.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
ATM (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line HepG2 using the antibody A300-135A. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 350.714.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford