1. Antibodies
  2. Homo sapiens
  3. ZSCAN29

ENCAB211EDR

Antibody against Homo sapiens ZSCAN29

Homo sapiens
K562, GM12878, HepG2
characterized to standards
Status
released
Source (vendor)
Bethyl Labs
Product ID
A303-284A
Lot ID
1
Characterized targets
ZSCAN29 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Aliases
michael-snyder:AS-1384
External resources

Characterizations

ZSCAN29 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody A303-284A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 96.719
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
ZSCAN29 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody A303-284A. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 96.719.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
ZSCAN29 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
compliant
Caption
IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using the antibody A303-284A, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitter comment
SKIV2L2 involves in mRNA splicing, rRNA processing. KIF2C is not transcription related protein. ILF3 was also detected in MS of ZSCAN29 (ENCAB552CAO). NOP56 is poly(A) RNA binding and snoRNA bindingprotein.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
ZSCAN29 (Homo sapiens)
GM12878
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878 using the antibody A303-284A. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 96.719.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
ZSCAN29 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2 using the antibody A303-284A. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 96.719.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford