ENCAB198KDI
Antibody against Homo sapiens CNOT7
Homo sapiens
HepG2
characterized to standards
Homo sapiens
any cell type or tissue
partially characterized
- Status
- released
- Source (vendor)
- MBLI
- Product ID
- RN110PW
- Lot ID
- 001
- Characterized targets
- CNOT7 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- External resources
Characterizations
CNOT7 (Homo sapiens)
compliant
- Caption
- Western blot following CRISPR against CNOT7 in HepG2 whole cell lysate using CNOT7 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CNOT7.CNOT7 protein appears as the green band, Tubulin serves as a control and appears in red.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U41HG009889
- Download
- CNOT7-HEPG2-CRISPR.png
CNOT7 (Homo sapiens)
HepG2
compliant
- Caption
- IP-Western Blot analysis of HepG2 whole cell lysate using CNOT7 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-CNOT7 antibody (lanes under 'CNOT7').
- Submitted by
- Steven Blue
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- HepG2_MBLI_RN110PW_001_CNOT7.png
CNOT7 (Homo sapiens)
not submitted for review by lab
- Caption
- IP-WB analysis of K562 whole cell lysate using CNOT7 specific antibody. Lane 1 is 2.5% of five million whole cell lysate Input, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal Anti-CNOT7 (CAF1) pAb. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
- Submitted by
- Balaji Sundararaman
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- MBLI_RN110PW_001_CNOT7.png