1. Antibodies
  2. Homo sapiens
  3. SIN3B

ENCAB194LUB

Alternate accession: ENCAB081CER

Antibody against Homo sapiens SIN3B

Homo sapiens
GM12878, K562, HeLa-S3, HepG2
characterized to standards
Status
released
Source (vendor)
Santa Cruz Biotech
Product ID
sc-13145
Lot ID
B2802
Characterized targets
SIN3B (Homo sapiens)
Host
mouse
Clonality
monoclonal
Isotype
IgG2b
Antigen description
raised against amino acids 172-228 of mSin3B of mouse origin
Aliases
michael-snyder:SIN3B-human_sc-13145_B2802, michael-snyder:AS-1129
External resources

Characterizations

SIN3B (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation of SIN3B from K562 cells using sc-13145. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with sc-13145. Lane 3: material immunoprecipitated using control IgG. Band A was excised from gel and subject to analysis by mass spectrometry. The expected band size is 133 kDa.
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
SIN3B (Homo sapiens)
GM12878K562HeLa-S3HepG2
Method: immunoblot
Attachment from submitter
Attachment from submitters
compliant
Caption
Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order: GM12878, K562, HeLaS3, HepG2 using the antibody sc-13145x. Molecular Weight: 133.066
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
SIN3B (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
compliant
Caption
IP followed by mass spectrometry: Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using sc-13145, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGE Bis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by Colloidial Coomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford