ENCAB190LDX
Antibody against Homo sapiens GABPA
Homo sapiens
K562
partially characterized
- Status
- released
- Source (vendor)
- Santa Cruz Biotech
- Product ID
- sc-22810
- Lot ID
- G0114
- Characterized targets
- GABPA (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Isotype
- IgG
- Antigen description
- GABP-α Antibody (H-180) is a rabbit polyclonal IgG provided at 200 µg/ml. Epitope corresponding to amino acids 1-180 mapping at the N-terminus of GABP-α of human origin
- Antigen sequence
- MTKREAEELIEIEIDGTEKAECTEESIVEQTYAPAECVSQAIDINEPIGNLKKLLEPRLQCSLDAHEICLQDIQLDPERSLFDQGVKTDGTVQLSVQVISYQGIEPKLNILEIVKPADTVEVVIDPDAHHAESEAHLVEEAQVITLDGTKHITTISDETSEQVTRWAAALEGYRKEQER
- Aliases
- michael-snyder:AS-1336
- External resources
Characterizations
GABPA (Homo sapiens)
K562
Method: immunoprecipitation
compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody sc-22810x. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
- Reviewer comment
- UniProt has the expected size at 51kD instead of 60kD.
- Submitted by
- Denis Salins
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- 1060_7_GABPa_sc-22810x_2ug.jpg
GABPA (Homo sapiens)
K562
Method: immunoprecipitation
not compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody sc-22810x. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
- Reviewer comment
- Multiple bands and already have a cleaner IP in K562 that is compliant.
- Submitted by
- Denis Salins
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- 1060_8_GABPa_sc-22810x_5ug.jpg